Composite
J13002AvOp

Part:BBa_K1648048:Design

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-05-31)


AvOprF induced by J13002


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 555


Design Notes

Insert the oprF into J13002 using standard restriction sites.

Source

J13002A is obtained from iGem distribution kits. The oprF gene was obtained by PCR from the Azotobacter vinelandii strain DJ genome.

References